Ashby Augustine

Senior Research Associate @ Bioplus Life Sciences

About

Expressed proteins in bacterial expression system and brought it up to 5L fermenter level  Experience in strain engineering for target protein expression and construction of novel metabolic pathways in microbial system  Expertise in vector construction, molecular cloning and transformation  Developed a pharmaceutical metabolite by In-vitro conversion of a biomolecule by means of enzymatic assay which is used for myopia  Experience in the development of novel strains using methods like random mutagenesis to engineer metabolic pathways in microbial system  Basic practical knowledge in CRISPR-Cas9 technology  Development and standardisation of multiplex PCR kit for infectious diseases (HBV,HCV,HIV and dengue)  Microbial identification by DNA barcoding (Contract Research Service) Bioinformatics tools: NCBI Blast, Clustal omega, Phylogenetic tree analysis by MEGA software  In-vitro maintenance of MTB culture and handling of different micro organisms  Immunization and Antibody development in rabbit and mice  Small scale and large scale production of molecular biology kits (Dispatched 450 RNA isolation kits for COVID-19)  Bio-conjugation (ASO-Latex conjugation, KLH-Peptide conjugation, HRP conjugation)  Preparation of Magnetic Biospheres for DNA and RNA isolation

Country

India

City

Bengaluru

Industry

Pharmaceuticals

Skill

Plasmid Isolation, Delivery Of Projects, Agarose Gel Electrophoresis, Fluorescence Microscopy, Computer Application Training, Gene Synthesis, Conference Coordination, In Vitro, Transformations, Immunization, Biodegradation, Research Support, Ion Chromatography, Analytical Biochemistry, Plasmid Transformation, Chromatography, DNA Isolation, Gel Extraction, Antimicrobial Resistance, DNA ligation

Experience

Senior Research Associate

Bioplus Life Sciences

2026-4 - Present · 6 mos

India

Metabolic Engineering

Bioplus Life Sciences

2022-2 - Present · 4 yrs 8 mos

Hosur

Expressed a two different polypeptides in bacterial expression system (E.coli) by screening of transformants and standardizing the expression conditions. Standardized the in-vitro assay for the conversion of biomolecule to a pharmaceutical metabolite used for myopia by using an enzyme. Expressed a human protein in yeast (Pichia pastoris) expression system and worked with its downstream processing. Experienced in the development of novel strains using methods like random mutagenesis to engineer metabolic pathways in microbial system.

Research associate

Bioplus Life Sciences

2022-2 - 2026-7 · 4 yrs 6 mos

Hosur, Tamil Nadu, India

Junior Scientist

Bhat Biotech (P) Ltd.com

2019-6 - 2022-2 · 2 yrs 9 mos

Karnataka, India

Junior Scientist

Bhat Biotech India Pvt. Ltd

2019-6 - 2022-2 · 2 yrs 9 mos

Developed and standardized multiplex PCR kits for infectious diseases such as HBV,HCV,HIV and dengue by using clinical samples. Identified microorganisms by DNA barcoding (Contract Research Services). Handled avirulent Mycobacterium for the initial development of diagnostic kit maintained the culture in-vitro. Experienced in immunization and antibody development in rabbit and mice. Basic knowledge in conjugating antibodies and peptides by Bio-conjugation technique - ASO-Latex conjugation, KLH-Peptide conjugation, HRP conjugation etc.

Intern

Bhat Biotech India Pvt. Ltd

2018-12 - 2019-5 · 6 mos

Project title: Laboratory scale expression and purification of Taq Polymerase enzyme. Supervisor: Dr. Mukti Nath Mishra, Head of Metabolic Engineering, Bioplus Life Sciences, Hosur, India. Description: The recombinant plasmid was transformed into a vector and expressed in bacteria. The culture conditions were standardized and the protein was purified by ion exchange chromatography followed by checking the functional activity.