Archana B

- Flow cytometry (multi-color), cell-based assays, ELISA - VCN analysis (TaqMan qPCR), IU titer assays (HEK293) - Viral vector & cell therapy: release and stability testing - Method development, qualification, and tech transfer (CTL/CDMO) - Technical writing: SOPs, protocols, reports - Data analysis & cross-functional team presentations

- Bioanalytical assay validation (ELISA, HPLC-SEC, SoftMax Pro) - Clinical sample analysis (Phase 1; >2000 samples) - Pharmacokinetics (PK) data analysis using GraphPad Prism - Instrument qualification (IQ/OQ/PQ) – HPLC, plate readers, washers - GLP/GMP lab operations & equipment management - Scientific writing: test methods, protocols, validation & stability reports - Automation: Biomek FXP/4000 for immunoassays - Cell-based potency assay development and tech transfer to CROs - Cross-functional collaboration and scientific presentations to leadership

- Flow Cytometry (FACSCalibur, Canto-II, MACSQuant) - Immunophenotyping & Cell Viability (CD34+, TruCount) - ELISA (IFN-γ, PEDF), CAR-T Potency Assays - Aseptic Cell Culture (AGS, K562, CAR-T, etc.) - Environmental Monitoring (EM, Media Fill, Microbial ID) - GMP QC Testing (In-Process & Final Product) - Equipment Calibration & Troubleshooting - Deviation/OOS Investigations, CAPA, Change Control - SOP Writing & Document Control - QC Training & Inventory Management

- In-vivo dosing & animal handling - Preclinical models (GVHD, lupus, IBD, MS, oncology) - Blood/tissue collection & analysis - Clinical chemistry & hematology - Behavioral phenotyping - IACUC protocol & SOP writing - PK/PD & transgenic studies - Equipment calibration & maintenance

- Teaching: Pharmacology, Anatomy & Physiology, Molecular Biology, Biochemistry - Lab instruction: Pharmacology, Microbiology, Molecular Biology - Academic seminar participation: Pharma innovation, patents, research ethics - Curriculum delivery & student mentoring - Educational workshop facilitation

- Responsible for taking lectures for Bachelor of Pharmacy students on the subject Pharmacology and toxicology, Human Anatomy & Physiology, Pathophysiology and community Pharmacy. - Actively Participated in following seminar: A) "International conference on "Nano-pharmaceutical Therapeutics: From Laboratory to clinical Practice" Poster presented : 1) Comparative study of anti-ulcer activity of marketed Ayurvedic Preparation(PCL-003). 2) Anti-arthritic Activity evaluation of Solanum erianthum (D.don) root bark in albino rats(PCL-006). 3) Anti-stresses Activity of Argyreia Speciosa roots in experimental animals(PCL-012) B) " GUJCOST sponsored international conference on "Emerging Trends in Drugs Research and Developement"

- Formulation & Development of Effervescent Tablets; flavor Masking (Cola Flavor) - Pharmaceutical Product Development - Patent Filing & Provisional Patent Experience

Courses: - Experimental methods in Molecular Biology. - DNA microarray techniques - Immunology - Biology of Cancer - Drug discovery - Drug development - GMP - GLP

During two years of journey I gained following hands on experience - PCR technology, qPCR - DNA isolation from Plant - RNA isolation from yeast - Various animal models in Pharmacology,animal handling and various route of administration - Bio- markers testing - ELISA - Western blotting/Southern blotting - Cell culture techniques - Paper and thin layer chromatography - Buffer solution preparation - Gel electrophoresis - NGS assay ,Sanger sequencing - Cell based assay (MTT assay), 96 well plate and pipetting