april rideout

* Performed raw materials and finished goods testing. * Adhered to SOPs in a cGMP environment supplemented with strict documentation. * Daily duties included: preparing and sterilizing media, pouring plates, collecting and plating both environmental and plant water samples. * Results were reported from plate counts to monitor for high or unusual results. * Utilized sterile technique at the bench and prepared serial dilutions.

* Challenged the ability of children to think critically by building specific devices out of LEGO bricks in response to a themed story. * Answered the concerns of customers with product knowledge. * Entertained children with in-store play activities to capture their interest with scenarios from films such as Lord of the Rings, The Avengers, and the Lone Ranger. * A clean and aesthetic store environment was maintained with concise attention to detail.

* Introduced undergraduate students to fundamental introductory microbiological techniques, including proper use of aseptic technique and the importance of maintaining a clean laboratory environment. * Duties included media and reagent prep, preparation of bacterial cultures, and overall laboratory setup for all sessions, as well as participation in grading of student assignments.

* Profiled the combined effects of physiological fluid shear and temperature on the growth and select pathogenesis-related stress responses of the enteric pathogen Salmonella bongori, including acid, oxidative, bile, and thermal stress. * Analyzed the growth profiles of several strains of Salmonella bongori in the rotating wall vessel (RWV) bioreactor under different physiological fluid shear levels and a range of temperatures. * Prepared RNA samples of S. bongori for use in quantitative real time PCR (qRT-PCR) gene expression studies. * Received training on an adherence and invasion assays using HT-29 human intestinal epithelial cells exposed to S. bongori.

* Maintenance and use of plasmid libraries of over 130 unique strains of E. coli. * Produced 130 unique expression strains of E. coli by co-transformation with multiple combinations of plasmids. * Mini and midi plasmid preps for purification of plasmid DNA. * Growth of E. coli cells and induction of expression of human proteins and RNA in E. coli cells. * TRAPeze assays of E. coli cell lysates, PCR reactions, determination of samples using polyacrylamide (PAGE) gel electrophoresis, and documentation and analysis using UVP gel imaging software. * Ordered supplies and maintained a working inventory to keep the laboratory running efficiently. * Trained students to use the UVP gel imaging software and shaking incubators in neighboring laboratories.

Activities and Societies: Vocal Ensemble, Concert Choir, Drama Club, Apollo Players, National Honor Society