Anne-Sophie Åstrand-Tournillon

Development of new affinity peptides targeting vascular adhesion molecules involved in angiogenesis and atherosclerosis. Main techniques: Cell culture (mammalian cell lines and primary cells), Phage display with immobilized protein and mammalian cells, Design of plasmids and cloning, ELISA, Production and purification of recombinants proteins (ÄKTA purification system), HPLC, flow cytometry, SPR (biacore), Stable transfections. Main task: - Laboratory work - Project management - Presentation of results - Writing and reviewing protocols - Collaboration with partners

Follow up on the PhD project, research on the p53/MDM2/MDM4 regulatory loops

Study of the regulatory loop between p53 and its two major regulators MDM2 and MDM4 Main techniques: Design of plasmids and cloning, Mutagenesis, PCR, RTqPCR, Cell culture (primary cells and mammalian cell lines), cell assays, Western Blot, ELISA (protein-protein and protein-RNA), In vitro translation, Production and purification of recombinants proteins (ÄKTA purification system), Sequencing, Immunolabeling, Transfection (transient and stable), Immunoprecipitation, S35 Pulse labelling experiment. Main task: - Laboratory work - Project management - Presentation of results - Supervising students - Collaboration with partners

Inserm UMRS 787, Early-onset myopathies: genetics, pathophysiology and search for new therapies

Inserm Technion, U898, DNA damage, cell survival and cancer stem cells Main techniques: Cell culture (stem cell), cell assays, western blot, RTqPCR, IHC.

Internship at the Professor Tak Mak Laboratory, Campbell Family institute for breast cancer research at the Princess Margaret hospital

Molecular and cellular physiology laboratory, IBGC-CNRS