Anna Faith Nguyen, PhD

· Represented the Protein Sciences Department in internal and partner programs, presenting novel data and collaborating cross-functionally to ensure alignment with project timelines. · Managed the development of new purification and analytical methods by overseeing a team of direct reports. · Enhanced and advanced quality control organizational systems, originally implemented in previous roles, by leading a team of direct reports to drive continuous improvements. · Was chosen to become Safety Officer, supporting on-site laboratory personnel in achieving laboratory and office safety/compliance standards. · Performed key Protein Sciences responsibilities, including protein purification, method development, quality control testing, and intact mass LC-MS analysis.

· Purified well over 500 proteins from 3 mL to 20 L scale protein productions of various antibody-based formats, complex bispecific formats, short half-life formats, cytokine-based formats, enzyme-based formats, and various antigens. · Developed and troubleshot various methods for protein purification and transient transfection. · Evaluated various chromatography columns and methods, as well as analytical testing techniques for more efficient and consistent protein purification. · Managed 3 passionate and dedicated direct reports, assisting in the development of their technical skills and overall career development. · Assumed the role of Safety and Lab Manager for the Protein Sciences department, driving a significant reduction or elimination of common safety violations within the first year. · Developed and implemented an intact mass LC-MS method and data analysis protocol, enabling the detection of quality deviations not identifiable by other analytical techniques.

• Utilized an electronic lab notebook as well as a protein filing system • Developed purification strategies for antibodies, probodies, bispecific antibodies, and antibodies of other formats from 30 mL to 60 L scale • Developed purification strategies for cytokines and cytokine-based proteins from 30 mL to 4 L scale • Produced and optimized production of antibody or cytokine derivatives for small-scale purifications utilizing tissue culture techniques such as mammalian cell culture, transfection, cell counting, sterile technique, and filtration • Utilized an automated workstation ( TECAN system) for purification of small scale preps (3-30 mL) • Purified antibody derivatives utilizing various chromatographic techniques (Protein A, Protein L, CH1, various SEC, Cation and Anion Exchange CEX/AIX/IEX, Anti-HSA, Anti-FLAG, Anti-Myc, CHT type 1 and 2, other Affinity, etc.) on AKTA instruments while minimizing endotoxin contamination • Utilized Tangential Flow Filtration (TFF) and optimized concentration techniques for various proteins, looking out for aggregation issues • Conducted endotoxin readings, analytical HPLC SEC chromatography, SDS-PAGE, chip-based capillary electrophoresis, UV-Vis readings, Octet titer readings, and interpretation of mass spectroscopy results in protein quality analysis • Performed quality control testing in all purified proteins before filing protein into an electronic online system • Formulated protein samples for various experiments, including for animal dosing • Generated Certificated of Analysis (CoA) for produced molecules for various studies • Gained experience with tandem, multi-column chromatography purification using the AKTA Versatile Valve system • Part of the Diversity & Inclusion Group, helping to spearhead the effort to help connect students to CytomX for career advice • Trained several new employees on various purification, transfection, and QA techniques • Acted as a manager to a direct report

· Established a small-scale production and purification protocol, significantly increasing throughput for screening compared to previous methods. · Produced, purified, and QC-tested proteins both in small scale (3-30 mL) and large scale (150-500 mL). · Designed and implemented a DNA plasmid organizational system and quality control protocol, drastically lowering protein production errors. · Streamlined quality control documentation for proteins and DNA used in R&D, improving the clarity and efficiency of product and data transfer between departments and with CROs. · Enhanced the in vivo dosing sample preparation protocol, streamlining communication and improving efficiency between the Protein Sciences department and other R&D teams.

-Summarized and wrote biochemistry lessons for university-level students. -Worked with Penn Foster teachers and professors in structuring lessons for university-level students. -Researched current biochemical advancements to add to lessons

Completed research and dissertation revolving around the use and design of CC Chemokine-based inhibitors of inflammation and HIV under Professor Patti J. LiWang utilizing biophysical, biochemical, molecular biological, microbiological, and virological techniques.

Prepared quizzes and homework assignments, helped create exams, graded work, prepared and presented presentations, and held office hours both in person and online for Biochemistry courses.

Completed research and dissertation revolving around the use and design of CC Chemokine-based inhibitors of inflammation and HIV under Professor Patti J. LiWang

Undergraduate research on acetylcholinesterase inhibitors and assay development was performed under the late Dr. Barry Wilson