Ankita Sharma, PMP®, PhD

• Lead project with clientele across the globe focused on oncology, immunology, neurological diseases with successful delivery of the targets. • Accountable for quality delivery on time for multiple projects value over $ 10 million. • Leading & driving project progress by ensuring timely deliverables, Gantt charts, budget management, preparing communication plan, meeting minutes, assisting in action and decisions planning • Planning & co-ordinating activities across various functional areas, monitoring resource management, and risk management at various stages of projects • Aiding stakeholders in effective and consistent communication to all stakeholders. • Spearheading effective client communication to facilitate project support and explore potential avenues for extension. • Improved client satisfaction by 85% • Increased returning client rate by 30% • Leading in-person meetings, site visits and presentation to highlight achievements and the overall project progress to audience of different levels.

-Lead scientist to assess anticancer potential of metallotheraproteins - Subject Matter Expert for Flow cytometry and functional bioassays - Established multi-parameter flow cytometry based intracellular and surface marker assays (e.g, transcriptional factors including STAT proteins, phosphorylated proteins, surface and apoptosis markers- Annexin, PI, 7AAD) - Expertise with FlowJO, CytExpert, MUSE for based flow data analysis - Evaluated drugs/ compounds for GPCRs via functional assays (cAMP, B-arrestin, Calcium flux) - Established assay for PBMCs induction and cytokine release for immune-modulation of drug compounds -Established PBMCs thawing and freezing protocols -Assessment of cytokines release post-drug incubation via magnetic beads based multiplex ELISA (>300 compounds screened) -Expertise with stable cells (CHO-K1- GPRCs) and multiple cancerous cells including leukemia (MV411, MOLM13, A529; epithelial cells (CHO, HEk293T), HeLa - Trained several new members and developed SOPs for various assays / techniques - Established spheroid (3D) cell culture assays for drug screening using Incucyte

Lead scientist for the project focusing on the role of immunogenetics in stress resiliency against pathogens and environmental challenges using transcriptomics and metabolomics approaches to establish a genetic association with resiliency. - Established circulatory serum miRNA transcriptome - Development of Knockout cells using CRISPR-Cas9 - Developed an immune stress model to study the innate immune response - Transcriptome analysis- RNASeq, PCR arrays (mRNA/miRNA) - Expertise in multiplex ELISAs and flowcytometry assays

-Lead Scientist for project collaborating with Defense research organization (India) to establish transcriptome of high altitude animals aiming to improve endurance in the hypobaric-hypoxic environment -Established in-vitro three-dimensional culture of mammary gland using mammary epithelial cells - Generated and analysed transcriptome profile of blood cells (PBMCs, Neutrophils) under heat-stressed and hypobaric-hypoxic stress both in-vivo and in-vitro to identify key immune and environemental stress markers

-Explored small microRNA to understand the mammary gland development using milk-derived epithelial cells from different lactation stages. - Well versed with qPCR and microarray assisted expression analysis, mammalian cell culture- 3D and 2D, cellular assays- apoptosis, proliferation, cytotoxicity and ELISAs

- Optimisation of transient transfection in insect and mammalian cell culture - Recombinant protein production at a large scale in mammalian cell culture using large bioreactors

- Teaching immunology and biotechnology courses to graduate students

Graduation and Masters