Alexandros Grammatikopoulos

-Served in the Medical Corps, in the 35𝐭𝐡 𝐌𝐞𝐝𝐢𝐜𝐚𝐥 𝐂𝐨𝐦𝐩𝐚𝐧𝐲, Tactical Command/21st Infantry Regiment "Drama", 16th Mechanized Infantry Division. -Transferred to the 424 𝐆𝐞𝐧𝐞𝐫𝐚𝐥 𝐌𝐢𝐥𝐢𝐭𝐚𝐫𝐲 𝐓𝐫𝐚𝐢𝐧𝐢𝐧𝐠 𝐇𝐨𝐬𝐩𝐢𝐭𝐚𝐥, in Thessaloniki, Greece, where he worked as an 𝐀𝐬𝐬𝐢𝐬𝐭𝐚𝐧𝐭 𝐚𝐭 𝐭𝐡𝐞 𝐛𝐥𝐨𝐨𝐝 𝐝𝐨𝐧𝐚𝐭𝐢𝐨𝐧 𝐝𝐞𝐩𝐚𝐫𝐭𝐦𝐞𝐧𝐭 and as an 𝐀𝐬𝐬𝐢𝐬𝐭𝐚𝐧𝐭 𝐚𝐭 𝐭𝐡𝐞 𝐦𝐢𝐜𝐫𝐨𝐛𝐢𝐨𝐥𝐨𝐠𝐲 𝐚𝐧𝐝 𝐛𝐢𝐨𝐜𝐡𝐞𝐦𝐢𝐬𝐭𝐫𝐲 𝐥𝐚𝐛𝐨𝐫𝐚𝐭𝐨𝐫𝐲 including the biochemistry, hematology, hemostasis coagulation, urochemistry, biological fluid culture and blood collection departments. 𝐓𝐚𝐬𝐤𝐬: • Carried out day-day-day duties accurately and efficiently. • Collected blood samples with focus on accuracy and adherence to aseptic techniques. • Maintained strict aseptic fields when collecting biological samples, minimizing staff and patient infection risks. • Analysed bodily fluids with laboratory equipment, detecting anomalies related to diseased states or acute injuries. • Involved in biological fluid cultures, blood grouping and cross matching of blood transfusion bottles. • Conducted diagnostic tests for SARS-CoV-2 tests. • Conducted on-call 24h laboratory duties. • Developed and maintained courteous and effective working relationships.

𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐠𝐫𝐨𝐮𝐩: Prof. Dr. Aphrodite Kapurniotu, Division of Peptide Biochemistry, Technical University of Munich, Germany. • Planned and executed experiments regarding the synthesis and purification of small peptide inhibitors. • Gathered, arranged and corrected research data to create representative graphs and charts highlighting results for presentations. • Set up equipment, organized inventory and maintained facilities. • Collaborated with team members to initiate best practices to achieve organizational goals. • Provided support in areas of cell culture and molecular biology, both effectively and on-time. • Collaborated with team to develop and implement best practices to accomplish all team goals • Presented our findings in conferences and meetings. 𝐌𝐞𝐭𝐡𝐨𝐝𝐬: Peptide Synthesis using Solid Phase Peptide Synthesis method (SPPS), peptide purification using RF-HPLC system, Fluorescence Spectroscopic Titration Studies.

𝐏𝐫𝐨𝐣𝐞𝐜𝐭: Identification and detection of mitochondrial mutations in tRNA genes and correlation to patients with Sarcoidosis and Idiopathic Pulmonary Fibrosis. 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐠𝐫𝐨𝐮𝐩: Prof. Konstantinos Gourgoulianis, MD, PhD, Professor of Respiratory Medicine, Faculty of Medicine, School of Health Sciences, University of Thessaly, Greece. • Organized and carried out the experimental part of the study with great emphasis on the genetic screening of local populations. • Good Clinical Practice (GCP) regulations and guidance compliance. • Proved successful working within tight deadlines and fast-paced atmosphere. • Used coordination and planning skills to achieve results according to schedule. • Exceeded goals through effective task prioritization and great work ethic. • Supervised and trained students. • Gathered, collected, edited and polished experimental results in order to present them in conferences and meetings. • Received the1st Clinical Research Award, issued by the Hellenic Thoracic Society. • Collaboration with multidisciplinary, international and high-structured research groups publishing 1 paper and 3 posters. 𝐌𝐞𝐭𝐡𝐨𝐝𝐬: DNA extraction from human whole blood, PCR, SSCP, Clean-up of PCR products, gel extraction of PCR products and shipping for sequencing, editing results of sequencing using bioinformatics software (Bioedit, BLAST, Mitomap, 1000 Genomes Browser).

𝐏𝐫𝐨𝐣𝐞𝐜𝐭: Identification and detection of mitochondrial mutations in tRNA genes and correlation to patients with Alzheimer’s Disease. 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐠𝐫𝐨𝐮𝐩: Ass. Prof. Emily Zifa, DEA, PhD, Assistant Professor of Biology and Neurobiology, Department of Biochemistry and Biotechnology, School of Health Sciences, University of Thessaly, Greece. • Planned, modified and executed research techniques in terms of genetic screening. • Completed research, compiled data, updated spreadsheets and produced timely reports. • Trained students. • Recorded data and maintained source documentation following good documentation practices. • Prepared literature for presentations and submissions to peer-review journals. • Performed statistical, qualitative and quantitative analysis. • Published 2 posters. 𝐌𝐞𝐭𝐡𝐨𝐝𝐬: DNA extraction from human whole blood, PCR, RFLP, SSCP, Clean-up of PCR products and shipping for sequencing, editing results of sequencing using bioinformatics software (bioedit, BLAST, Mitomap, 1000 Genomes Browser).

Funded by the Operational Programme "Education and Lifelong Learning" (NSRF 2007-2013). • Assigned to the biochemistry laboratory of the hospital. • Collected blood samples from the outpatient clinics, using aseptic techniques. • Performed laboratory routine exams in biochemical analysers. • Carried out day-day-day duties in both efficient and accurate manners.

𝐃𝐢𝐬𝐬𝐞𝐫𝐭𝐚𝐭𝐢𝐨𝐧 𝐭𝐨𝐩𝐢𝐜: Molecular mechanisms and peptide-based inhibitors of protein interactions in aging diseases. 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐠𝐫𝐨𝐮𝐩: Prof. Dr. Aphrodite Kapurniotu, Division of Peptide Biochemistry, Technical University of Munich, Germany. • Designed, synthesized, purified and biophysically characterized small peptide inhibitors against neurodegenerative diseases (AD and T2D). • Developed, optimized, documented, presented, planned and implemented experiments. • Project leadership; Strategic and critical thinking, Troubleshooting, Problem solving. • Supervised and trained laboratory personnel. • Maintained laboratory instruments. • Published 1 paper and 5 posters. 𝐌𝐞𝐭𝐡𝐨𝐝𝐬: Peptide Synthesis-Solid Phase Peptide Synthesis method (SPPS), peptide purification using RF-HPLC system, Mass Spectrometry, UV-Vis/Fluorescence spectroscopy, circular dichroism (CD), Aggregation kinetic assays (ThT), cell cultivation and viability methods (MTT), ELISA, Slot blots.

𝐓𝐡𝐞𝐬𝐢𝐬 𝐭𝐨𝐩𝐢𝐜: Development of experimental model studying the effects of smoking on ENaC in sheep tracheal epithelium (2014). 𝐑𝐞𝐬𝐮𝐥𝐭𝐞𝐝 𝐢𝐧: 2 posters. 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐠𝐫𝐨𝐮𝐩: As. Prof. Sotirios Zarogiannis, M.Sc., PhD, Associate Professor of Physiology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Greece. • Performing in situ experiments, using electrophysiology systems. • Primary cell cultures. • Plan, conduct, present and document experiments. • Organizing, editing and polishing experimental results. 𝐌𝐞𝐭𝐡𝐨𝐝𝐬: Isolation and preparation of epithelial and mesothelial specimens (trachea, pericardium, pleura) from different animals (sheep, pig and rabbit), operation of Ussing apparatus, bioinformatics software (UCSC Genome Bioinformatics, Primer3 Input, Ensembl genome Browser, Graphpad prism version 5 and 6), primer design, Western Blot, Spectrophotometry, Primary cell cultures of sheep epithelial cells.

𝐓𝐡𝐞𝐬𝐢𝐬 𝐭𝐨𝐩𝐢𝐜: Detection of mitochondrial mutations in tRNA genes of Asn, Cys, Tyr, Ser (UCN) and Asp in patients with Alzheimer’s disease (2013). 𝐑𝐞𝐬𝐮𝐥𝐭𝐞𝐝 𝐢𝐧: 2 posters. 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐠𝐫𝐨𝐮𝐩: Ass. Prof. Emily Zifa, DEA, PhD, Assistant Professor of Biology and Neurobiology, Department of Biochemistry and Biotechnology, School of Health Sciences, University of Thessaly, Greece. • Genetic screening. • Plan, conduct, present and document experiments. • Organizing, collecting, polishing and present experimental results. • Completion of the experimental part of the project. 𝐌𝐞𝐭𝐡𝐨𝐝𝐬: DNA extraction from human whole blood, PCR, RFLP, SSCP, Clean-up of PCR products and shipping for sequencing, editing results of sequencing using bioinformatics software (bioedit, BLAST, Mitomap, 1000 Genomes Browser).