Aishwarya V.

• Developed in vitro model using CRISPR tools for validating the development candidate for target indication. • Leading target specific research project, with focus on developing innovative treatments for vascular malformations. Program involves-  Development of stable disease model cell lines using advanced molecular biology techniques such as CRISPR/Cas9 and lentiviral transductions, for target validation and bi-specific Ab drug discovery.  Designing and implementation of assays, including AlphaLISA, immunodetection, FACS, gene expression analysis and similar signaling pathway readouts to establish functional assays for drug evaluation. • Excellent communication and presentation skills, with experience presenting in both research group settings and company-wide meetings.

• Lead investigator for a therapeutic program (undisclosed indication). Independently designed and screened guides in in vitro models. Designed ELISA and JESS assays to detect therapeutically relevant protein levels in in vitro and in vivo models, alongside qPCR and NGS based assays (in collaboration with Computational team) for genotyping studies. o Completed in vivo testing at mouse and NHP level. Proficient in handling mouse and NHP samples for immuno-detection and genotyping. o Routinely generated and presented data with timelines in the project meeting involving multiple teams- Editing Technologies, In vivo Pharmacology, Computational biology, and IP. • Currently involved in the evaluation of donor templates as a gene correction platform. This project involves large scale lipid-based transfection optimization in multiple cell lines for large donor template insertion such as plasmids, along with mRNA and sgRNA. Reviewing literature and incorporating new reagents to trouble shoot protocol to improve integration efficiency. o Performing ddPCR assays as one of the downstream analyses to read successful integration, other than FACS, EVOS Imaging, cytokine- expression and long-range sequencing. o As a sub-project, I am invested in developing a protocol for the synthesis of a donor template in-house (a cost-effective and efficient platform for template synthesis) • Trained in research level LNP formulations. • Experienced in managing direct report (Co-op) for more than one project. Clearly elucidating complex projects/ technical know-hows in a simple and straight-forward manner were an important aspect in this role. • Routinely maintaining accurate and detailed records of all experiments and project timelines for accountability and IP purposes.

• Standardized and optimized transfection procedures to multiplex edit in primary T cells. Screened numerous guides across various loci to determine the best multiplexing scenario. The success with multiplex editing impacted the team goal, thereby expanding the scope of project to identify novel proteins for precision-based gene editing approach. o Also, trained members from cross functional teams on the above platform to implement the multiplex editing strategy towards specific programs. • Implemented and evaluated reporter-based landing pad screens through extensive optimization with the goal of enabling high-through put screen for the precision-based editor platform. The reporter-based cell lines were generated using both adherent and suspension cells and screened using antibiotic selection and flow cytometry. Cell banks were eventually maintained cell banks for each line. • Involved in production of lentiviral vectors for screening novel protein for precision-based in a high-through put manner. Transduced cell lines with lentiviral vectors to generate stable cell lines with the protein of interest. • Proficient in research grade mRNA synthesis and process optimization, followed by QC and analysis using fragment analyzer. Improved the existing process of mRNA synthesis making it more time efficient. • Trained several new team members with majority of the cell biology workflow protocols, assays, and instrumentations in CRISPR Tx lab. • Presented research and development findings in quarterly portfolio reviews, exploratory science forums, weekly group meetings, journal clubs and to external partners wherever involved.

• Assisting with invention evaluation process • Performing primary and secondary market research, preparing non-confidential summaries & memos and assist with maintaining databases • Supporting business development activities

- As a part of the platform research team, I helped develop their platform technology by building assays for better data outputs and conducted in-vitro studies in human and mouse tissue cultures. - My research contributions majorly consisted of method development for Crispr Cas 9 detection (ex-vivo) and performing in-vitro validation studies to study the efficiency of the protein. - Assay development vastly involved techniques such as - Western blot standardization, PCR, Guide screens and Multi color Flow cytometry - In-vitro studies involved mammalian cell culture, reporter mouse tissue culture, co-incubation and nucleofection techniques, followed by flow cytometry (with bead compensation) derived output.

• Assisting with invention evaluation process • Performing primary and secondary market research, preparing non-confidential summaries & memos and assist with maintaining databases • Supporting business development activities

Delineating tumor-lymphatic interaction in inflammed micro-environment, specifically pertaining to Head and neck squamous cell carcinoma. 1. Determined the clock gene expressions in the inflammed LEC's 2. Studied the clock gene expression in non-metastatic and metastatic head and neck cancer cell lines. 3. Alterations in EMT genes in HNSCC cell lines in response to inflammed LECs. Techniques involved- HNSCC cell culture, RT-PCR to study EMT and clock genes, Western blotting.

• I was a team member of the global project - "Angels Initiative". • Independently worked with hospitals (Private and Government Institutions) to set up Standard Operating Procedures which helped in reducing the Door-to-Needle for treatment of stroke patients. • The established protocols reduced existing time to treat in hospitals and impacted several patients’ functional outcome post therapy.

• Responsible for identifying mechanisms in radio-resistant breast cancer cell lines to make the radiation therapy more effective. • Studied the involvement of cytokines, DNA damage and Telomeres in radio-resistant reast cancer cells. • Also studied colony forming characteristics of normal and irradiated cancer cell lines.

• Training on processing samples for Quality testing in pharmaceutical firm. • Methods: Analytical & Microbiology lab testing.