Angela Kennedy-Curran

• Independently executed a recurring 2-week assay with iPSC-derived cardiomyocyte models to generate potency data, influencing preclinical candidate selection for $424M Bayer collaboration • Troubleshot challenging immunofluorescence protocol, restoring assay functionality and enabling team to meet critical deliverables for Bayer collaboration • Utilized medium- and small-volume liquid handlers to develop and perform cell-based and molecular assays such as FISH/IF, CellTiter-Glo, and qPCR, including compound treatments and transfections, in 384- or 1536-well plate formats • Assisted with generation of stable cell line model for DM1 secondary assay through colony picking, characterization of clone phenotypes by FISH, and expansion of desired clone • Analyzed and interpreted complex data and presented results to diverse audiences, including meetings with Biology department and external Pharma collaborators; documented experiments accurately in electronic lab notebook

• Advanced drug discovery collaboration with Pfizer on Myotonic Dystrophy Type 1 (DM1) by assisting with development, miniaturization, and execution of a high-throughput immunofluorescence screening assay on an integrated robotic system • Designed experiments investigating DM1 disease pathology in cellular models to inform secondary assay selection; performed secondary assays including fluorescent in-situ hybridization (FISH) and RT-PCR

• Evaluated compound inhibitory potential using a biochemical luminescence assay, analyzed data with MATLAB, and documented experiments thoroughly and accurately in lab notebook

• Expedited completion of a high-throughput screening (HTS) pilot by helping to optimize a biochemical luminescence assay and run 10,000 compounds • Assisted in developing an ELISA assay to measure small molecule activity • Supported lab operations through inventory tracking and ordering assistance

• Developed 8 novel MSD assays for 3 potential drug targets, laying foundations for an important new quantitative assay within the company • Cultured multiple mammalian cell lines to screen optimal lysis protocols for use in MSD assay development • Ran MSD assays on various cell and tissue samples from discovery biology group to aid in understanding of multiple disease models

• Investigated expression levels of ANTXR1/2 in human tissues by designing and optimizing primers and running RT-qPCR • Discovered co-expression patterns of ANTXR2 with various markers in mouse dorsal root ganglia by performing RNAscope in-situ hybridization and brightfield/fluorescence microscopy • Characterized expression levels of ANTXR2 in novel mouse conditional knockout strain via IP injection of tamoxifen to induce recombination, dorsal root ganglia dissection, RNA extraction, and RT-qPCR • Facilitated behavioral and infection studies by weaning, genotyping, weighing, shaving, and monitoring mice

• Aided drug discovery research by conducting controlled-duration, in vitro cannabinoid stability assays followed by HPLC analysis to assess metabolic stability of compounds in various matrices • Assisted researchers by preparing buffers and reagents, silanizing well plates and vials, and sterilizing equipment

• Took on several administrative duties and projects, including procuring missing invoices for reconciliation, compiling a contact directory, and organizing office space

• Appointed approx. 3-6 crew members to positions each shift to maximize customer satisfaction and profit and mediated between customers and upper management • Completed security and maintenance tasks needed to open and close the store • Prepared and delivered deposits, documented results of safe counts and solved monetary discrepancies

• Served approx. 100 customers per hour during busy periods • Maintained cleanliness and functionality of restaurant

"Life Sciences, Research and Culture" Spring 2020 Semester study abroad program in Dundee, Scotland. Conducted lab research project on transposon mutagenesis in B. subtilis investigating gene function in bacterial phenotype and biofilm formation using molecular cloning and sequencing techniques. Produced a mock manuscript with publication-style figures.